| 质粒名称: | pBABE-zeo largeTgenomic |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 1778 |
| 存储实验室: | Bob Weinberg |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-zeo |
| 载体出品公司: | -- |
| 空载体大小: | 4800 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 博莱霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | large T and small T antigen,large T genomic DNA,LTg,sv40gp6 |
| 物种来源: | simian virus |
| 插入大小: | 2100 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI（未销毁） |
| 3’克隆位点: | BamHI（未销毁） |
| 5’测序引物 | pBABE 5' |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE-zeo largeTgenomic was a gift from Bob Weinberg (Addgene plasmid # 1778 ; http://n2t.net/addgene:1778 ; RRID:Addgene_1778) |
| 参考文献部分: | Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Mol Cell Biol 2002 Apr;22(7):2111-23. 10.1128/MCB.22.7.2111-2123.2002 PubMed 11884599 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE-zeo largeTgenomic 7388 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABE-zeo largeTgenomic
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7388)
AUTHORS Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM,
DeCaprio JA, Weinberg RA
TITLE Enumeration of the simian virus 40 early region elements necessary
for human cell transformation.
JOURNAL Mol Cell Biol 2002 Apr;22(7):2111-23.
PUBMED 11884599
REFERENCE 2 (bases 1 to 7388)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7388
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 9..478
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 543..742
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 943..1359
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1289..1311
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1327..1343
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS join(1446..1691,2038..3918)
/codon_start=1
/product="SV40 (simian virus 40) large T antigen"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
intron 1692..2037
/label=large T intron
/note="SV40 (simian virus 40) large T antigen intron"
primer_bind complement(3994..4014)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3999..4328
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4179..4314
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4241..4260
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4338..4712
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
LTR 4883..5352
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5482..5499)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5653..6241)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5733..5752)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6401..7261)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7024..7043
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7262..7366)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
61 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
121 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
181 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
241 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
301 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
361 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
421 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
481 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
541 gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
601 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
661 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc
721 cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat
781 gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt
841 tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt
901 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact
961 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
1021 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
1081 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
1141 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
1201 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
1261 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
1321 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg cggcctctga
1381 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttg
1441 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
1501 ttgaaaggag tgcctggggg aatattcctc tgatgagaaa ggcatattta aaaaaatgca
1561 aggagtttca tcctgataaa ggaggagatg aagaaaaaat gaagaaaatg aatactctgt
1621 acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc tgactttgga ggcttctggg
1681 atgcaactga ggtatttgct tcttccttaa atcctggtgt tgatgcaatg tactgcaaac
1741 aatggcctga gtgtgcaaag aaaatgtctg ctaactgcat atgcttgctg tgcttactga
1801 ggatgaagca tgaaaataga aaattataca ggaaagatcc acttgtgtgg gttgattgct
1861 actgcttcga ttgctttaga atgtggtttg gacttgatct ttgtgaagga accttacttc
1921 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
1981 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
2041 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
2101 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
2161 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
2221 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
2281 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
2341 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
2401 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
2461 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatcc attttctgtt
2521 attgaggaaa gtttgccagg tgggttaaag gagcatgatt ttaatccaga agaagcagag
2581 gaaactaaac aagtgtcctg gaagcttgta acagagtatg caatggaaac aaaatgtgat
2641 gatgtgttgt tattgcttgg gatgtacttg gaatttcagt acagttttga aatgtgttta
2701 aaatgtatta aaaaagaaca gcccagccac tataagtacc atgaaaagca ttatgcaaat
2761 gctgctatat ttgctgacag caaaaaccaa aaaaccatat gccaacaggc tgttgatact
2821 gttttagcta aaaagcgggt tgatagccta caattaacta gagaacaaat gttaacaaac
2881 agatttaatg atcttttgga taggatggat ataatgtttg gttctacagg ctctgctgac
2941 atagaagaat ggatggctgg agttgcttgg ctacactgtt tgttgcccaa aatggattca
3001 gtggtgtatg actttttaaa atgcatggtg tacaacattc ctaaaaaaag atactggctg
3061 tttaaaggac caattgatag tggtaaaact acattagcag ctgctttgct tgaattatgt
3121 ggggggaaag ctttaaatgt taatttgccc ttggacaggc tgaactttga gctaggagta
3181 gctattgacc agtttttagt agtttttgag gatgtaaagg gcactggagg ggagtccaga
3241 gatttgcctt caggtcaggg aattaataac ctggacaatt taagggatta tttggatggc
3301 agtgttaagg taaacttaga aaagaaacac ctaaataaaa gaactcaaat atttccccct
3361 ggaatagtca ccatgaatga gtacagtgtg cctaaaacac tgcaggccag atttgtaaaa
3421 caaatagatt ttaggcccaa agattattta aagcattgcc tggaacgcag tgagtttttg
3481 ttagaaaaga gaataattca aagtggcatt gctttgcttc ttatgttaat ttggtacaga
3541 cctgtggctg agtttgctca aagtattcag agcagaattg tggagtggaa agagagattg
3601 gacaaagagt ttagtttgtc agtgtatcaa aaaatgaagt ttaatgtggc tatgggaatt
3661 ggagttttag attggctaag aaacagtgat gatgatgatg aagacagcca ggaaaatgct
3721 gataaaaatg aagatggtgg ggagaagaac atggaagact cagggcatga aacaggcatt
3781 gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat
3841 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
3901 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt ggatcccagt gtggtggtac
3961 gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
4021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
4081 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
4141 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
4201 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
4261 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
4321 tttgcaaaaa gcttaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
4381 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
4441 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
4501 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
4561 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
4621 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
4681 gcgtgcactt cgtggccgag gagcaggact gaccgatcga taaaataaaa gattttattt
4741 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
4801 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
4861 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
4921 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
4981 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
5041 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
5101 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
5161 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
5221 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
5281 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
5341 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
5401 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
5461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
5521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
5581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
5641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
5701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
5761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
5941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
6001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
6061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
6121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
6181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca
6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
6421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
6481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
6541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
6601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
6661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
6721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
6781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
6841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
6901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
6961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
7021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
7081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
7141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
7201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
7261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
7321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
7381 aagtgcca
//
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